Review



e coli lemo21 de3 cells  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    New England Biolabs e coli lemo21 de3 cells
    E Coli Lemo21 De3 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/bio_rxiv__64898__2026__03__06__710107-228-5-9
    Average 96 stars, based on 390 article reviews
    e coli lemo21 de3 cells - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau
    Article Snippet: .. The cDNA encoding the 6×His-tagged 2N4R isoform of human tau was cloned into the pRK172 vector (a gift from the Feinstein lab, UCSB) and expressed in LEMO21 cells (NEB #C2528J). .. For preparing phosphomimetic tau mutants, site-directed mutagenesis was performed on the wild-type plasmid via overlap-extension PCR (primer sequences provided in Supplementary Data ), and the resulting products were assembled into full-length plasmids using Gibson assembly (NEB, #E2611S).

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.
    Article Snippet: .. The cDNA encoding the 6×His-tagged 2N4R isoform of human tau was cloned into the pRK172 vector (a gift from the Feinstein lab, UCSB) and expressed in LEMO21 cells (NEB #C2528J). .. For preparing phosphomimetic tau mutants, site-directed mutagenesis was performed on the wild-type plasmid via overlap-extension PCR (primer sequences provided in Supplementary Data 1), and the resulting products were assembled into full-length plasmids using Gibson assembly (NEB, #E2611S).

    Article Title: Tau condensation on DNA and localization on centromeres: A potential link to cell division
    Article Snippet: .. The 2N4R isoform of 6×His-tagged human tau protein was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). ..

    Article Title: Structuring Role of Tau-Tubulin Co-Condensates in Early Microtubule Organization
    Article Snippet: .. Full-length human tau protein with a 6x His-tag was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). ..

    Plasmid Preparation:

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau
    Article Snippet: .. The cDNA encoding the 6×His-tagged 2N4R isoform of human tau was cloned into the pRK172 vector (a gift from the Feinstein lab, UCSB) and expressed in LEMO21 cells (NEB #C2528J). .. For preparing phosphomimetic tau mutants, site-directed mutagenesis was performed on the wild-type plasmid via overlap-extension PCR (primer sequences provided in Supplementary Data ), and the resulting products were assembled into full-length plasmids using Gibson assembly (NEB, #E2611S).

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.
    Article Snippet: .. The cDNA encoding the 6×His-tagged 2N4R isoform of human tau was cloned into the pRK172 vector (a gift from the Feinstein lab, UCSB) and expressed in LEMO21 cells (NEB #C2528J). .. For preparing phosphomimetic tau mutants, site-directed mutagenesis was performed on the wild-type plasmid via overlap-extension PCR (primer sequences provided in Supplementary Data 1), and the resulting products were assembled into full-length plasmids using Gibson assembly (NEB, #E2611S).

    Article Title: Tau condensation on DNA and localization on centromeres: A potential link to cell division
    Article Snippet: .. The 2N4R isoform of 6×His-tagged human tau protein was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). ..

    Article Title: Structuring Role of Tau-Tubulin Co-Condensates in Early Microtubule Organization
    Article Snippet: .. Full-length human tau protein with a 6x His-tag was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). ..

    Transformation Assay:

    Article Title: A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis
    Article Snippet: .. This was transformed into LEMO21 cells (New England Biolabs), grown to OD 600 of 0.6–0.8 at 37 °C in autoinducing TB media before overnight induction at 25 °C. ..

    Article Title: Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter
    Article Snippet: .. Constructs were transformed into either E. coli BL21(DE3) or Lemo21 cells (New England Biolabs) and grown to an OD 600 of 0.6–0.8 at 37°C in autoinducing TB medium before overnight induction at 25°C. .. Cells were harvested and resuspended in 20 m M Tris–HCl pH 8, 1 μg ml −1 DNaseI and PMSF, followed by lysis by cell disruption (Constant Systems) at 172 MPa pressure and centrifugation at 14 000 rev min −1 for 20 min (45 Ti rotor, Beckman).

    Article Title: Design of cell-type-specific hyperstable IL-4 mimetics via modular de novo scaffolds.
    Article Snippet: Huilin Yang, Umut Y. Ulge, Alfredo Quijano-Rubio, Zachary J. Bernstein, David R. Maestas, Jung-Ho Chun, Wentao Wang, Jian-Xin Lin, Kevin M. Jude , Srujan Singh , Brian T. Orcutt-Jahns , Peng Li, Jody Mou, Liam Chung, Yun-Huai Kuo, Yasmin H. Ali, Aaron S. Meyer, Warren L. Grayson, Nicola M. Heller , K. Christopher Garcia , Warren J. Leonard, Daniel-Adriano Silva , Jennifer H. Elisseeff , David Baker & Jamie B. Spangler

    Construct:

    Article Title: Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter
    Article Snippet: .. Constructs were transformed into either E. coli BL21(DE3) or Lemo21 cells (New England Biolabs) and grown to an OD 600 of 0.6–0.8 at 37°C in autoinducing TB medium before overnight induction at 25°C. .. Cells were harvested and resuspended in 20 m M Tris–HCl pH 8, 1 μg ml −1 DNaseI and PMSF, followed by lysis by cell disruption (Constant Systems) at 172 MPa pressure and centrifugation at 14 000 rev min −1 for 20 min (45 Ti rotor, Beckman).

    Expressing:

    Article Title: Design of cell-type-specific hyperstable IL-4 mimetics via modular de novo scaffolds.
    Article Snippet: Huilin Yang, Umut Y. Ulge, Alfredo Quijano-Rubio, Zachary J. Bernstein, David R. Maestas, Jung-Ho Chun, Wentao Wang, Jian-Xin Lin, Kevin M. Jude , Srujan Singh , Brian T. Orcutt-Jahns , Peng Li, Jody Mou, Liam Chung, Yun-Huai Kuo, Yasmin H. Ali, Aaron S. Meyer, Warren L. Grayson, Nicola M. Heller , K. Christopher Garcia , Warren J. Leonard, Daniel-Adriano Silva , Jennifer H. Elisseeff , David Baker & Jamie B. Spangler



    Similar Products

    e coli lemo21 de3 cells  (New England Biolabs)
    96
    New England Biolabs e coli lemo21 de3 cells
    E Coli Lemo21 De3 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/bio_rxiv__64898__2026__03__06__710107-228-5-9
    Average 96 stars, based on 1 article reviews
    e coli lemo21 de3 cells - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    competent e coli lemo21 de3 cells  (New England Biolabs)
    96
    New England Biolabs competent e coli lemo21 de3 cells
    (A) Aerobic UQ 8 biosynthesis pathway in <t>E.</t> <t>coli</t> and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.
    Competent E Coli Lemo21 De3 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/bio_rxiv__64898__2026__03__05__709815-207-12-17
    Average 96 stars, based on 1 article reviews
    competent e coli lemo21 de3 cells - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    virus strains lemo21 de3 e coli cells new england biolabs cat c2528j  (New England Biolabs)
    96
    New England Biolabs virus strains lemo21 de3 e coli cells new england biolabs cat c2528j
    (A) Aerobic UQ 8 biosynthesis pathway in <t>E.</t> <t>coli</t> and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.
    Virus Strains Lemo21 De3 E Coli Cells New England Biolabs Cat C2528j, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/pm41790558-244-7-12
    Average 96 stars, based on 1 article reviews
    virus strains lemo21 de3 e coli cells new england biolabs cat c2528j - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    lemo21 de3 cells  (New England Biolabs)
    96
    New England Biolabs lemo21 de3 cells
    (A) Aerobic UQ 8 biosynthesis pathway in <t>E.</t> <t>coli</t> and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.
    Lemo21 De3 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/pm41580424-248-16-18
    Average 96 stars, based on 1 article reviews
    lemo21 de3 cells - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    lemo21 cells  (New England Biolabs)
    96
    New England Biolabs lemo21 cells
    (A) Aerobic UQ 8 biosynthesis pathway in <t>E.</t> <t>coli</t> and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.
    Lemo21 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/pmc12855940-385-26-28
    Average 96 stars, based on 1 article reviews
    lemo21 cells - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    escherichia coli lemo21 de3 cells  (New England Biolabs)
    96
    New England Biolabs escherichia coli lemo21 de3 cells
    (A) Aerobic UQ 8 biosynthesis pathway in <t>E.</t> <t>coli</t> and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.
    Escherichia Coli Lemo21 De3 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lemo21+cells/Lemo21(DE3)+Competent+E%2E+coli/pm41232797-72-17-22
    Average 96 stars, based on 1 article reviews
    escherichia coli lemo21 de3 cells - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Aerobic UQ 8 biosynthesis pathway in E. coli and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.

    Journal: bioRxiv

    Article Title: UbiB proteins mediate an ATP-dependent decarboxylation step in bacterial ubiquinone biosynthesis

    doi: 10.64898/2026.03.05.709815

    Figure Lengend Snippet: (A) Aerobic UQ 8 biosynthesis pathway in E. coli and putative alternative decarboxylation step. OPP would be exported from the membrane to the soluble Ubi-complex by UbiB to be modified in UQ 8 . (B) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (WT) and Δ ubiX Δ ubiD cells grown aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The chromatograms are representative of results from three independent experiments. The peaks corresponding to OHB, UQ 8 , DMK 8 , MK 8 and UQ 10 , as a standard, are indicated. (C-D) OHB content (MS peak aera, mass detection M-H with m/z 681.3, C) and UQ 8 content (ECD detection, D) in cells grown either aerobically (+O 2 ) or anaerobically (-O 2 ) in LB medium. The structure of each quantified compound is shown. Values are shown with means ± SD (n=3). Proteins belonging to the Ubi-complex are in framed. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid, UQ 8 , ubiquinone 8, DMK 8 , demethylmenaquinone, MK 8 , methylmenaquinone and UQ 10 , ubiquinone 10. ND: not detected.

    Article Snippet: The overexpression of E. coli MBP-UbiB ΔC47 was carried out in chemically competent E. coli Lemo21(DE3) cells (New England Biolabs), transformed with the pETM40-MBP-UbiB ΔC47 plasmid.

    Techniques: Membrane, Modification

    (A) UQ 8 (ECD detection), (B) OPP (MS peak area, mass detection M+NH 4 + , m/z 656.5) and (C) OHB (MS peak aera, mass detection M-H, m/z 681.5) contents of lipid extracts from E. coli WT strain MG1655 and Δ ubiXc , Δ ubiB and Δ ubiX Δ ubiB mutant strains. Values are shown with means ± SD (n=3). The structure of each compound quantified is shown. ND: not detected.

    Journal: bioRxiv

    Article Title: UbiB proteins mediate an ATP-dependent decarboxylation step in bacterial ubiquinone biosynthesis

    doi: 10.64898/2026.03.05.709815

    Figure Lengend Snippet: (A) UQ 8 (ECD detection), (B) OPP (MS peak area, mass detection M+NH 4 + , m/z 656.5) and (C) OHB (MS peak aera, mass detection M-H, m/z 681.5) contents of lipid extracts from E. coli WT strain MG1655 and Δ ubiXc , Δ ubiB and Δ ubiX Δ ubiB mutant strains. Values are shown with means ± SD (n=3). The structure of each compound quantified is shown. ND: not detected.

    Article Snippet: The overexpression of E. coli MBP-UbiB ΔC47 was carried out in chemically competent E. coli Lemo21(DE3) cells (New England Biolabs), transformed with the pETM40-MBP-UbiB ΔC47 plasmid.

    Techniques: Mutagenesis

    (A) Representation of the conservation of the signature sequence and selected residues over 4011 UbiB protein sequences as obtained with WebLogo3 ( www.xeblogo.threeplusone.com ). The numbering corresponds to the residues of the E. coli protein. (B) Three-dimensional model of the active site pocket of UbiB from E. coli generated with AlphaFold 3. An ATP molecule, shown in wheat, was docked into the active site using AlphaFold 3. Secondary structures of the model are represented in grey by PyMOL. Highly conserved residues are represented as sticks, with oxygen atoms in red and nitrogen atoms in dark blue. Backbone color is associated to the data shown in panels (C) and (D). UQ 8 content in Δ ubiB (C) or Δ ubiX Δ ubiB (D) mutated strains transformed with plasmids expressing UbiB variants. Percentages referred to in the main text are indicated. In pink, mutation with minor or without effect in the two genetic backgrounds; in yellow, mutation which increases UQ 8 content only in Δ ubiX Δ ubiB strain; in blue, mutations which cause a strong decrease of the UQ 8 content mainly in Δ ubiX Δ ubiB strain; in green, mutations which cause a strong decrease of the UQ 8 content in the two genetic backgrounds. Values are shown with means ± SD (n=3). ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant by unpaired Student’s test comparing to WT UbiB. ND: not detected. (E) The proposed functions of the UbiB protein in the UQ biosynthetic pathway and the amino acids suggested as important for each function. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid; OPP, octaprenylphenol; UQ 8 , ubiquinone 8. ND: not detected.

    Journal: bioRxiv

    Article Title: UbiB proteins mediate an ATP-dependent decarboxylation step in bacterial ubiquinone biosynthesis

    doi: 10.64898/2026.03.05.709815

    Figure Lengend Snippet: (A) Representation of the conservation of the signature sequence and selected residues over 4011 UbiB protein sequences as obtained with WebLogo3 ( www.xeblogo.threeplusone.com ). The numbering corresponds to the residues of the E. coli protein. (B) Three-dimensional model of the active site pocket of UbiB from E. coli generated with AlphaFold 3. An ATP molecule, shown in wheat, was docked into the active site using AlphaFold 3. Secondary structures of the model are represented in grey by PyMOL. Highly conserved residues are represented as sticks, with oxygen atoms in red and nitrogen atoms in dark blue. Backbone color is associated to the data shown in panels (C) and (D). UQ 8 content in Δ ubiB (C) or Δ ubiX Δ ubiB (D) mutated strains transformed with plasmids expressing UbiB variants. Percentages referred to in the main text are indicated. In pink, mutation with minor or without effect in the two genetic backgrounds; in yellow, mutation which increases UQ 8 content only in Δ ubiX Δ ubiB strain; in blue, mutations which cause a strong decrease of the UQ 8 content mainly in Δ ubiX Δ ubiB strain; in green, mutations which cause a strong decrease of the UQ 8 content in the two genetic backgrounds. Values are shown with means ± SD (n=3). ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant by unpaired Student’s test comparing to WT UbiB. ND: not detected. (E) The proposed functions of the UbiB protein in the UQ biosynthetic pathway and the amino acids suggested as important for each function. Abbreviations: OHB, octaprenyl-hydroxybenzoic acid; OPP, octaprenylphenol; UQ 8 , ubiquinone 8. ND: not detected.

    Article Snippet: The overexpression of E. coli MBP-UbiB ΔC47 was carried out in chemically competent E. coli Lemo21(DE3) cells (New England Biolabs), transformed with the pETM40-MBP-UbiB ΔC47 plasmid.

    Techniques: Sequencing, Generated, Transformation Assay, Expressing, Mutagenesis